Trypsin digestion in immunoperoxidase staining.
نویسندگان
چکیده
منابع مشابه
Immunoperoxidase staining of leptospires.
Leptospires were detectable by light microscopy when Formalin-fixed preparations were reacted by direct and indirect methods with horseradish peroxidase-labeled specific antibody.
متن کاملKinetics of Trypsin Digestion
The anomalies of enzyme action have frequently been accounted for by the assumption of an intermediate compound between the enzyme and the substrate. I t was shown, however, by Nelson and Hitchcock t that in the case of invertase the experiments could not be accurately calculated on this basis. The writer found with trypsin' that although the effect of varying the substrate concentration could ...
متن کاملImproved method for trypsin digestion of Paraplast sections before immunofluorescence staining.
Formalin-fixed, Paraplast-embedded tissue sections mounted on subbed slides were digested with crude trypsin in modified Sorensen phosphate buffer containing sodium fluoride before immunofluorescence staining. This method prevented detachment of sections from slides during processing, thereby permitting immunofluorescence and histological examinations of adjacent serial sections.
متن کاملThe kinetics of trypsin digestion.
The present study of a typical proteolytic enzyme extends the quantitative investigations previously reported from this laboratory on the effects of pressure on protein systems in viva (1) and in vitro (2). This paper presents new data on the self-digestion of trypsin and the digestion of casein by trypsin; a forthcoming paper will discuss the effects of temperature and hydrostatic pressure on ...
متن کامل240 Kinetics of Trypsin Digestion
The anomalies of enzyme action have frequently been accounted for by the assumption of an intermediate compound between the enzyme and the substrate. I t was shown, however, by Nelson and Hitchcock t that in the case of invertase the experiments could not be accurately calculated on this basis. The writer found with trypsin' that although the effect of varying the substrate concentration could ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Histochemistry & Cytochemistry
سال: 1980
ISSN: 0022-1554,1551-5044
DOI: 10.1177/28.1.6153193